RNAi
Important to the understanding of a protein's function is observing what occurs to an organism when the protein is absent. A common way to inhibit the function of a protein is to stop its formation at the level of its mRNA transcript, usually by RNA interference. More can be read about this method here.
Results of silencing GLO1 have not been characterized in the model organisms C. elegans, D. melanogaster, or D. rerio, but the Saccharomyces Genome Database has documented several descriptions of GLO1 mutants. Yeast lacking GLO1 function are viable, but are subject to reactive oxidants that damage DNA.
Results of silencing GLO1 have not been characterized in the model organisms C. elegans, D. melanogaster, or D. rerio, but the Saccharomyces Genome Database has documented several descriptions of GLO1 mutants. Yeast lacking GLO1 function are viable, but are subject to reactive oxidants that damage DNA.
Chemical Genetics
GLO1 has been a subject of interest in medicine due to its overexpression in cancerous cells. Inhibition of the GLO1 has been shown to slow cell growth [2], indicating that it may be a target for drug therapy in cancer patients. Chemical inhibitors of GLO1 have been identified by analyzing the protein's 3D structure, and are shown below.
Microarray Data
A microarray is a biological assay to reveal different levels of gene expression and protein presence in various tissues or types of cells. It is useful to determine where a protein of interest is active, and how active it is. Gene Expression Omnibus is the NCBI database for microarray data, and holds experiments on GLO1 expression observed in muscle, brain, and other tissues for differing experimental purposes.
References
[1] Breslow DK, et al. (2008) A comprehensive strategy enabling high-resolution functional analysis of the yeast genome. Nat Methods 5(8):711-8
[2] Santarius, T., Bignell, G. R., Greenman, C. D., Widaa, S., Chen, L., Mahoney, C. L., Butler, A., Edkins, S., Waris, S., Thornalley, P. J., Futreal, P. A. and Stratton, M. R. (2010), GLO1—A novel amplified gene in human cancer. Genes Chromosom. Cancer, 49: 711–725. doi: 10.1002/gcc.20784
[3] Al-Balas Q, Hassan M, Al-Oudat B, Alzoubi H, Mhaidat N, Almaaytah A. Generation of the First Structure-Based Pharmacophore Model Containing a Selective “Zinc Binding Group” Feature to Identify Potential Glyoxalase-1 Inhibitors. Molecules. 2012; 17(12):13740-13758.
[2] Santarius, T., Bignell, G. R., Greenman, C. D., Widaa, S., Chen, L., Mahoney, C. L., Butler, A., Edkins, S., Waris, S., Thornalley, P. J., Futreal, P. A. and Stratton, M. R. (2010), GLO1—A novel amplified gene in human cancer. Genes Chromosom. Cancer, 49: 711–725. doi: 10.1002/gcc.20784
[3] Al-Balas Q, Hassan M, Al-Oudat B, Alzoubi H, Mhaidat N, Almaaytah A. Generation of the First Structure-Based Pharmacophore Model Containing a Selective “Zinc Binding Group” Feature to Identify Potential Glyoxalase-1 Inhibitors. Molecules. 2012; 17(12):13740-13758.
Site created by: Emma Baar
Last updated: 5-14-2013
University of Wisconsin - Madison
Last updated: 5-14-2013
University of Wisconsin - Madison